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        您的位置: 網(wǎng)站首頁 > 技術(shù)文章 > 人繆勒管抑制物質(zhì)/抗繆勒管激素(MIS/AMH)ELISA試劑盒英文說明書

        人繆勒管抑制物質(zhì)/抗繆勒管激素(MIS/AMH)ELISA試劑盒英文說明書

        發(fā)布日期: 2012-02-15
        瀏覽人氣: 2193

        Human MIS/AMH

         
        FOR RESEARCH USE ONLY
         
        Assay range0.8ng/ml - 24ng/ml                96determinations
        Purpose
        This kit allows for the determination ofMIS/AMH concentrations in Humanserum, cellculture supernates and other biological fluids
         
        Principle of the assay
        The kit assay Human MIS/AMHlevel in the sampleuse Purified Human MIS/AMHantibody to coat microtiter plate wells, make solid-phase antibody, then addMIS/AMHto wells,CombinedMIS/AMH antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Compley,Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed,reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration ofHuman MIS/AMHin the samples is then determined by comparing the O.D. of the samples to the standard curve.
        Materials provided with the kit

        1
        wash solution
        20ml×1bottle
        7
        Stopp Solution
        6ml×1 bottle
        2
        HRP-Conjugate reagent
        6ml×1 bottle
        8
        Standard(48ng/ml)
        0.5ml×1 bottle
        3
        Microelisa stripplate
        12well×8strips
        9
        Standard diluent
        1.5ml×1bottle
        4
        Sample diluent
        6ml×1 bottle
        10
        Instruction
        1
        5
        Chromogen Solution A
        6ml×1 bottle
        11
        Closure plate membrane
        2
        6
        Chromogen Solution B
        6ml×1 bottle
        12
        Sealed bags
        1

        Specimen requirements
        1.       extractas soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
        2.       Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
        Assay procedure
        1.       Dilute and add sample:Dilute Original density Standard as follow table:

        24ng/ml
        5 Standard
        150μl Original density Standard+150μl Standard diluent
        12ng/ml
        4 Standard
        150μl 5 Standard+150μl Standard diluent
        6ng/ml
        3 Standard
        150μl 4 Standard+150μl Standard diluent
        3ng/ml
        2 Standard
        150μl 3 Standard +150μl Standard diluent
        1.5ng/ml
        1 Standard
        150μl 2 Standard +150μl Standard diluent

        2.add sampleSet blank wells separay (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
        3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37.
        4.Configurate liquid: 30-fold(or 20-fold) wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
        5.washingUncover Closure plate membrane, discardLiquid, dry by swing, add washing buffer to every well, still for 30s then drain,repeat 5 times, dry by pat.
        6.add enzymeAdd HRP-Conjugate reagent 50μl to each well, except  blank well.
        7.incubateOperation with 3.
        8.washingOperation with 5.
        9.colorAdd Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37
        10.Stop the reactionAdd Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
        11.assaytake blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
        Steps description

        Standard, Sample diluent

         

        AddStandard, Sample diluent, incubate for 30 min at 37.

         

        Wash 5 time,AddHRP-Conjugate reagent, incubate for 30 min at 37.

         

        Wash 5 times,Add Chromogen Solution A and B, incubate for 30 min at 37.

         

        AddStopp Solution

         

        Read absorbance at 450nm within 15 min

         

        calculate

        Calculate
        Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
        Important notes
        1.       The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
        2.       washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
        3.       add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 min, if the number of sample is much , recommend to use Volley .
        4.       if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluenteand multiplied by the dilution factor.(×n×5).
        5.       Closure plate membrane only limits the disposable use, to avoid cross-contamination.
        6.       The substrate evade the light preservation.
        7.       Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
        8.       All samples, washing buffer and each kind of reject should according to infective material process.
        9.       Do not mix reagents with those from other lots.
         
        Storage and validity
        1Storage 2-8℃.
        2validity six months
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