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        您的位置: 網(wǎng)站首頁 > 產(chǎn)品中心 > > > bs-1252R一抗-多抗 Rabbit Anti-API5 antibody凋亡抑制因子5抗體
        • 一抗-多抗 Rabbit Anti-API5 antibody凋亡抑制因子5抗體
        產(chǎn)品名稱:

        一抗-多抗 Rabbit Anti-API5 antibody凋亡抑制因子5抗體

        產(chǎn)品品牌: SOLARBIO/索萊寶 價(jià)格:
        廠商性質(zhì): 生產(chǎn)廠家 產(chǎn)品時(shí)間: 2024-07-09
        一抗-多抗 Rabbit Anti-API5 antibody凋亡抑制因子5抗體
        貨號(hào):bs-1252R
        規(guī)格:0.1ml/0.2ml
        產(chǎn)品應(yīng)用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 (石蠟切片需做抗原修復(fù))
        not yet tested in other applications.

        產(chǎn)品概述

        品牌SOLARBIO/索萊寶

         一抗-多抗 Rabbit Anti-API5 antibody凋亡抑制因子5抗體

        產(chǎn)品編號(hào)bs-1252R
        英文名稱API5
        中文名稱凋亡抑制因子5抗體
        別    名apoptosis inhibitor 5; apoptosis inhibitory protein 5; API5; AAC-11; AAC11; API5L1; AAC 11; Antiapoptosis clone 11 protein; API5 like 1; Cell migration inducing gene 8 protein; Fibroblast growth factor 2 interacting factor 2; FIF; Migration inducing protein MIG8; MIG 8; MIG8; Protein XAGL; API5_HUMAN.
        研究領(lǐng)域腫瘤  免疫學(xué)  細(xì)胞凋亡  
        抗體來源Rabbit
        克隆類型Polyclonal
        交叉反應(yīng)Human, Mouse, Rat, Dog, Pig, Cow, Horse, Guinea Pig, 
        產(chǎn)品應(yīng)用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 (石蠟切片需做抗原修復(fù)) 
        not yet tested in other applications.
        optimal dilutions/concentrations should be determined by the end user.
        分 子 量57kDa
        細(xì)胞定位細(xì)胞核 細(xì)胞漿 
        性    狀Lyophilized or Liquid111
        濃    度1mg/1ml
        免 疫 原KLH conjugated synthetic peptide derived from human API5
        亞    型IgG
        純化方法affinity purified by Protein A
        儲(chǔ) 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
        保存條件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
        生 產(chǎn) 商BIOSS
        PubMedPubMed
        產(chǎn)品介紹background:
        Apoptosis inhibitor 5 (API5) is an antiapoptotic factor which may have a role in protein assembly. API5 expression is often upregulated in tumor cells, particularly in metastatic cells. It is a critical determinant of E2F1-induced apoptosis, acting downstream of E2F to suppress E2F-dependent apoptosis without generally blocking E2F-dependent transcription. Alternate Names: AAC 11; AAC11; Antiapoptosis clone 11 protein; API 5; API5; API5 like 1; API5L1; Cell migration inducing gene 8 protein; Fibroblast growth factor 2 interacting factor 2; FIF; MIG 8; MIG8; Migration inducing protein MIG8; Protein XAGL.

        Function:
        Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.

        Subunit:
        Monomer. Interacts with FGF2 and ACIN1.

        Subcellular Location:
        Nucleus. Cytoplasm. Note=Mainly nuclear. Can also be cytoplasmic. 
        Isoform 3: Cytoplasm.

        Tissue Specificity:
        Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer.

        Post-translational modifications:
        Acetylation at Lys-251 impairs antiapoptotic function.

        Similarity:
        Belongs to the API5 family.

        Gene ID:
        8539

        Database links:

        Entrez Gene: 8539 Human

        Entrez Gene: 11800 Mouse

        Omim: 609774 Human

        SwissProt: Q9BZZ5 Human

        SwissProt: O35841 Mouse

        Unigene: 435771 Human

        Unigene: 181824 Mouse

        Unigene: 402557 Mouse



        Important Note:
        This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

        IAP-5是細(xì)胞凋亡的一種抑制因子、參與細(xì)胞凋亡調(diào)控,可以用于各種惡性腫瘤的細(xì)胞凋亡的研究.
        產(chǎn)品圖片

        Antigen: bs-1252P, 0.2ug/100ul 
        Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; 
        Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; 
        TMB(C-0024) staining; 
        Read the data in MicroplateReader by 450nm. 

        Protein: mouse heart lysates(A); mouse pancreas lysates(B); 
        Primary: Anti-API5 (bs-1252R) at 1:300; 
        Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000; 
        ECL excitated the fluorescence; 
        Predicted band size : 57 kD
        Observed band size :57 kD

        每一種抗體可以分別標(biāo)記20種不同的標(biāo)記物:

        Alexa Fluor 350 APCCy7 HRP
        Alexa Fluor 488Biotin FITC PE 
        Alexa Fluor 555 Cy3 Gold RBITC
        Alexa Fluor 647 Cy5.5  PE-Cy3PE-CY7
        AP Cy5 PE-CY5PE-CY5.5 

        一抗-多抗 Rabbit Anti-API5 antibody凋亡抑制因子5抗體訂購說明:

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